Rapid method for identification of transgenic fish zygosity
نویسندگان
چکیده
منابع مشابه
ZyFISH: A Simple, Rapid and Reliable Zygosity Assay for Transgenic Mice
Microinjection of DNA constructs into fertilized mouse oocytes typically results in random transgene integration at a single genomic locus. The resulting transgenic founders can be used to establish hemizygous transgenic mouse lines. However, practical and experimental reasons often require that such lines be bred to homozygosity. Transgene zygosity can be determined by progeny testing assays w...
متن کاملPCR method for genotyping and zygosity-testing of RasH2 transgenic mice.
In short-term carcinogenicity testing using CB6F1-TgrasH2 mice, sibling nonTgrasH2 mice are used as a negative control. However, selection of TgrasH2 and nonTgrasH2 mice has been performed by PCR with only transgene specific primers by the conventional method. Therefore, the conventional method involves the risk of false negative results due to reaction failure, and contamination with TgrasH2 m...
متن کاملTurbo FISH: A Method for Rapid Single Molecule RNA FISH
Advances in RNA fluorescence in situ hybridization (RNA FISH) have allowed practitioners to detect individual RNA molecules in single cells via fluorescence microscopy, enabling highly accurate and sensitive quantification of gene expression. However, current methods typically employ hybridization times on the order of 2-16 hours, limiting its potential in applications like rapid diagnostics. W...
متن کاملRapid identification of clinical relevant minor histocompatibility antigens via genome-wide zygosity-genotype correlation analysis.
PURPOSE Identification of minor histocompatibility antigens (mHag) with classic methods often requires sophisticated technologies, determination, and patience. We here describe and validate a nonlaborious and convenient genetic approach, based on genome-wide correlations of mHag zygosities with HapMap single-nucleotide polymorphism genotypes, to identify clinical relevant mHags within a reasona...
متن کاملIdentification of the first Transgenic Aquatic Animal in Iran by PCR-Based Method and Protein Analysis
In the recent years, there is evidence of training a red type of zebrafish which differs from wild-type in body color. There is not any document how it reaches to the ornamental fish farms of Iran but at first, it was a doubt it belongs to a morphotype or genetic modification (GM). First of all, a set primer was designed to validate zebrafish species. Mitochondrial 16srDNA was selected and ampl...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Jurnal Akuakultur Indonesia
سال: 2007
ISSN: 2354-6700,1412-5269
DOI: 10.19027/jai.6.177-182